1.How is the Quadruple Marker Test Performed?

The Quadruple Marker Test is a basic blood test. To perform the test, a phlebotomist (a

healthcare professional trained to draw blood) will collect a blood sample from a vein in your

arm. The process only takes about 5–10 minutes and is generally quick and straightforward.

Once the blood sample is taken, it is sent to a laboratory for testing. The laboratory checks the

levels of four substances found in your blood. These substances are:

1. Alpha-fetoprotein (AFP): A protein produced by the baby’s liver. High levels can

indicate neural tube defects or abdominal wall anomalies, while low levels may suggest

an increased risk for Down syndrome.

2. Unconjugated estriol (uE3): A hormone produced by both the placenta and the fetus.

Low levels of uE3 can suggest an increased risk for Down syndrome.

3. Human chorionic gonadotropin (hCG): The placenta produces a hormone. A higher

risk for Down syndrome may be indicated by levels that are higher than normal.

4. Inhibin-A: A protein produced by the ovaries and placenta. Elevated levels may indicate

a higher risk of Down syndrome.

2.Why is the Quadruple Marker Test Important?

The test provides information about the likelihood that the baby may have certain genetic

disorders or birth defects. Notably, the Quadruple Marker Test is a screening test rather than a

diagnostic one. This means it does not diagnose any condition but helps estimate the risk based

on the levels of these substances in the blood.

If the results suggest a higher risk, further tests, like an ultrasound or amniocentesis, may be

recommended to get a clearer diagnosis

3.Is the Test Safe?

Yes, the Quadruple Marker Test is completely safe for both the mother and the baby. Since it

only involves drawing a blood sample, there are no risks involved in the procedure itself.